CHAPTER 29 Pentose Phosphate Pathway OBJECTIVES: 1. Describe the pentose phosphate pathway Names: Functions: Substrates: Product: Control Enzymes: Regulation: Compartment(s): Tissues of interest: 2. If a cell needs both NADPH and ribose-5-phosphate, what substrates from glycolysis are used by the pentose phosphate pathway to make them? If a cell needs only ribose-5-phosphate what substrates from glycolysis are used by the pentose phosphate pathway? 3. What is NADPH used for? 4. Assume that you have a patient that has hemolytic anemia a result of eating fava beans. Name the deficient enzyme and the other enzymes and products in the pathway that leads to the reduction of HOOH. 5. Concerning Al Martini: This patient has glucose-6-phosphate dehydrogenase deficiency. Why are red blood cells lysed while other cells of the body remain intact? Why didn't this disease show up earlier in life? KEYWORDS: anabolic pathway, antimalarial drugs, biosynthesis, cytosol, detoxification, fava beans, fructose-6-phosphate, glucose-6-phosphate, glucose-6-phosphate dehydrogenase, glutathione, glutathione peroxidase, glutathione reductase, glyceraldehyde-3-phosphate, glycolysis, H2O2, hexose monophosphate shunt, irreversible, NADPH, nonoxidative, oxidative, oxidized glutathione, pentose phosphate pathway, 6-phosphogluconate dehydrogenase, reduced glutathione, reductive biosynthesis, reversible, ribose-5-phosphate, pentose phosphate pathway, peroxide, primaquine, product inhibition, reactive oxygen species (ROS), sulfa drugs, sulfhydryl group, superoxide ion, vitamin C, vitamin E, ASSIGNMENTS: Only Section C. The Pentose Phosphate Pathway and Al Martini are assigned. Examine question 29.2, page 537. Examine only question 3 and not 1, 2, 4, and 5 at the end of Chapter 29. Understand the meaning of the key words in the context of Chapter 29. Practice Questions for Chapter 29 Objectives No Other Help REFERENCE: Marks' Basic Medical Biochemistry: A Clinical Approach, 2nd Edition, 2004, Williams and Wilkins (ISBN: 0-7817-2145-8) by Colleen M. Smith PhD, Allan D. Marks MD, and Michael A. Lieberman PhD